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Sterilization, Do i need to do it if...
Davodian
post May 7 2007, 03:45 AM
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Hi guys

I should be getting a kit soon and because i'm merely a student without alot of money i was planning to get one from ebay...i know you guys think they're trash but i'm learning so i'm just going to throw some money on a machine and some needles and what not. Has anyone bought a machine off ebay? and if so are they decent to work with?

And about sterilization...please don't flame me for being ignorant but i just wanted to know, when using disposable tubes and needles, can i re-use them if i'm only planning to practice on fake skin or pig skin or fruits? Sorry if its a moronic question...I know tubes and needles are cheap anyways and its not worth saving couple of dollars and risking infection but with regards to fruit and fake skin can i re-use dispoable or metal tubes without Proper sterilisation. Ofcourse when i do practice on myself or others disposables are one time use only DEFINITELY!

Thanks in advance.

Dave


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TattooTina
post May 7 2007, 04:15 AM
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I keep a tube and needle separate from the rest of my equipment that I use on fake skin "the rubber" kind". I keep them clean even though they are only for the fake skin and nothing else. They are also marked with colored tape and says PRACTICE on it, so I know what it was used for. I wouldn't recommend using the same needle on pig skin though, because its going to hold bacteria from the pig and you don't want that anywhere near your sterile equipment. I guess if you have to reuse your practice needle and tube keep them clean and in a sealed container away from the sterile stuff. Just to prevent any cross contamination.
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jOE bLOW
post May 7 2007, 04:19 AM
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It's good that as beginners you guys are thinking of things like this. That's the difference between a scratcher and an amateur if you ask me. :)


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Davodian
post May 7 2007, 04:31 AM
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Sweet!
Thanks Tina, thats a great idea to seperate the tube and needle i'll be using on fruit and fake skin from the others. Simple and safe. Ofcourse i will be doing my best to keep that needle and tube clean even though i am only using it on fake skin and fruits.

Cheers.

Dave


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Rhyno69
post May 7 2007, 06:23 AM
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I do what Tiny Tina does.  I bought a plastic toothbrush tavel tube and stuffed one end with cotton and thats where I keep my "melon needles". 
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DimebaG
post May 7 2007, 11:23 AM
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But don't forget, when you do do that don't forget to clean the needle and tube so you don't mix the colors....


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gurgigirl
post May 7 2007, 01:32 PM
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hi davodian,
just to say that for a cheaper sterilization set up it's possible to pick up an ultrasonic cleaner for about £15 and a pressure cooker (about £25) to steam sterilize your tubes - not a good idea to use it for needles, but okay as a basic setup for other bits. It's a good idea to read up on various sterilization methods (and what health departments require). An autoclave is ideal but the pressure cooker (to the best of my knowledge) will do much the same thing - for the future if you get the cash (an autoclave)  with a drying cycle is recommended although by far the priciest option! As previously said keep a track of what bits are used for practice skins etc
and try to get into good practice - keeping it clean is part of the deal, so it's good to start early.
best wishes
gurgigirl
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deadhands
post May 7 2007, 02:02 PM
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its a good idea to keep a needle n tube, just 4 the pig skin, and a set 4 fake skin. when i started out i got a wierd film grow on some of my fake skin because of the Bacteria in the pig skin  :o it wasent very nice to say the lest.
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citymorgue
post May 8 2007, 09:35 AM
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I do what Tiny Tina does.  I bought a plastic toothbrush tavel tube and stuffed one end with cotton and thats where I keep my "melon needles". 


Good idea ..i'm gonna do that. :D


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tattoobox
post May 8 2007, 11:33 AM
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I FOUND THIS ARTICLE IF IT HELPS


3.5 Methods Of Disinfection And Sterilization
Disinfectants

Disinfectants and "cold-sterilization" solutions destroy many
microorganisms (bacteria, viruses, fungi) but do not destroy bacterial
spores. Disinfection does not replace autoclave sterilization.

Disinfectants should be used on hard surfaces (ie. trays, countertop,
chair) and reusable, non-autoclaveable tools, such as plastic
calipers, before and after each procedure. Product usage instructions
vary and some may not be suitable for all surfaces or
applications. Some disinfectants are toxic and require special
disposal, making them inappropriate for soaking jewelry prior to
insertion.

Ultrasonic Cleaner

Prior to sterilization, instruments contaminated with blood and other
debris should be cleaned in an ultrasonic cleaner. The ultrasonic
cleaner uses cavitation (implosion of microscopic air bubbles produced
by ultrasonic vibration) to remove debris.

The ultrasonic cleaner is usually filled with a disinfectant or
ultrasonic-specific solution.

Jewelry should not be placed directly in the ultrasonic cleaner or
with tools or other jewelry because it will be scratched.

Sterilization

Sterilization is the process of killing all microorganisms including
bacteria, bacterial spores, fungi, and viruses. Improper sterilization
can result in the spread of infectious bacteria and bloodborne viruses
such as Hepatitis and HIV.

Boiling is not an appropriate method of sterilization.

Autoclave

The most complete and reliable method of sterilization is the steam
autoclave or chemical autoclave. The steam autoclave uses pressurized
steam; the chemical autoclave often uses Ethylene oxide. Steam
autoclaves are used by piercers more frequently than chemical
autoclaves because of the hazards associated with Ethylene oxide.

Pressure Cookers

Pressure cookers may also be used for sterilization but with many
disadvantages. Pressure cookers are not as reliable as autoclaves in
maintaining temperature and pressure and do not display time,
temperature or pressure (PSI). Pressure cookers will not dry the
sterilized items; wet sterilization bags are susceptible to
contamination.

If a pressure cooker is used, items should be processed for at least
30 minutes at 15-20 pounds pressure. Internal indicators which
indicate that heat and steam have penetrated the packaging and reached
the instruments should be used inside every sterilization bag.

Dry Heat

While Occupational Safety and Health Administration Standards
(December 6, 1991; 29 CFR 1910.1030) recognizes dry heat sterilizers,
they have several disadvantages. Sterilizing items in a dry heat
sterilizer is extremely time and energy consuming. Some jewelry and
piercing equipment cannot tolerate the high temperature required for
sterilization.

Sterilization Packaging

Instruments to be sterilized are placed in self-sealing sterilization
bags or sterilization tubing sealed with indicator tape. The tape and
the outside of the bag or tubing are marked with external indicators
which change color when exposed to heat and steam. Internal indicators
which indicate that heat and steam have penetrated the packaging and
reached the instruments can be used as an added precaution.

Sterilization bags should be allowed to dry thoroughly before being
removed from the autoclave. Wet bags are susceptible to contamination.

Sterilizer Monitoring and Testing

Sterilizers should be spore tested at least monthly. Spore testing
kits contain two bacteria spore samples, one of which is cycled in the
autoclave and the other used as a control. Spore testing systems are
available for in-studio testing or testing by an independent
laboratory.


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gurgigirl
post May 9 2007, 06:31 AM
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that's a great bit of info tattoobox...  (I was starting to worry about suggesting a pressure cooker )
thanks and best wishes
gurgigirl  ;D
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tattoobox
post May 9 2007, 12:33 PM
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ive read alot of articles and spoke to people and in my research ive found that a pressure cooker is an accepted way of sterilization
you will have to leave it cooking for 2-3 times as long as an autoclave,
the only diffrence is that it wont dry your equipment if in pouches/bags
so basically you either have to use straight away or store in some sort of sterilized enviroment.
but as for needles i dont sterilize and dont use them again
if i made my own needles then i would sterilize them before use anyway.
alot of authorities insist on an autoclave in your shop and to be spore tested once a month at least
and some authorities wont even ask questions on your methods
bearing in mind im from the UK.
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Mario AKA mark
post May 9 2007, 12:40 PM
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Sterilization packages are plastic and paper when the paper is still wet form being processed it’s considered a permeable membrane. After I sterilize I vent the autoclave it releases pressure and pushes all the water back into the reservoir and lets the pressure out. I then crack the door and all the steam escapes in ten minutes the bags are dry from the 265-degree heat leaving the chamber.

You need to have your packages paper side up or if you are doing a large load they need to be bundles with their sides up. If you use a pressure cooker type you need to vent the chamber and take the top off and move to the side like the lid on a pot. The steam will escape drying your packages and not compromising the items. When you don’t see any more steam coming out and everything looks dry then remove from chamber


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DimebaG
post May 9 2007, 04:43 PM
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ChadDoom
post May 16 2007, 12:54 AM
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ok, let me make sure I got this straight, how do you use a pressure cooker to sterelize, I get that you by the test strips and bags, but do you allow the to sit in the water of the pressure cooker or do you have a platform the sit on so they are only exposed to steam?
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tattoobox
post May 16 2007, 03:30 AM
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no dont put them in water theres usually a tray that sits on top of the water ,place them there
the steam under pressure will do the job just fine, i  recommend to leave for about 30 minutes as opposed to 10-15 in an autoclave
then look at what mark said earlier about the steam clearing the pot
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Tripp
post May 16 2007, 04:52 AM
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hey D, as for the ebay tat gun, I got one when i first started but also got tubes and needles from a tattoo supplier. Then I upgraded to a pro gun and over all was much better. But for starting off i shouldnt see why not with the ebay tat gun.


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seenoblithe
post Jun 1 2007, 01:57 PM
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just to let you know when saving money,  if you buy a steel grip for like ten or 15 back and buy gripless disposable tubes, they are like 30 cents a piece as opposed to the tubes with grips that are 75 cents to $1.00... how much money are you looking to throw down if you dont mind me asking.... because if you shop right you can get a decent kit for the same amount as the $250 ones on ebay
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SirTralala
post Jun 10 2007, 11:13 AM
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Hi, just wanted to tell some informations I have about sterilization and pressure cookers.
Pressure cookers use a similar way to sterilize, but the problem with the cookers is that, before the steri-process starts, there should be a vakuum inside the cooker and a pressure cooker can`t provide this. If you don`t have the vacuum, there could be little "security-shields" made of air wrapped around possible bacterias, and so the steam can`t kill them.
To really kill all the bad stuff that could be on equipment you should sterilize at 138C° , about 2.5 bar at least 6 minutes (better to take a safety time of 12 minutes) - sorry about the C° and bar measurements, I am from Austria/Europe, we have a different system there, but I  hope I could help. :)


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TattooingDragon
post Jun 18 2007, 06:30 PM
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Ok I have been mostly reading and reading and reading some more but, I have a question for anyone of whom can answer this for me. First, I have on there way both an autoclave and an ultrasonic machine. (one can never be too careful I believe). When I was practicing as a cosmetologist we used a chemical called Quaternary Ammonium Compounds  (Quats) as a part of our sanitation regimine.

The following is some information about Quats:

Quats Are The Best Choice: They Provide  Safe and Effective One-Step Cleaning and Disinfecting
Quaternary Ammonium Chlorides or “quats” as they are commonly known are based upon the active ingredient benzalkonium chloride. These quaternary salt compounds can be formulated with a variety of ingredients to provide a safe and effective neutral pH, disinfectant-cleaner without damaging floor finishes or sensitive floor surfaces.  In addition, quats are economical and extremely effective odor control agents when used according to label directions.   

Quats are effective in destroying a broad spectrum of harmful microorganisms. They are effective in killing the following microorganisms while cleaning the surfaces upon which they reside – all in one simple step.

1.                   Gram negative and gram positive bacteria like salmonella typhi, staphylococcus aureus, streptococcus epidermidis and pseudomonas aeruginosa,

2.                   viruses like HIV-1, Herpes simplex 1 and 2

3.                   Antibiotic resistant strains of bacteria including methicillin resistant staphylococcus aureus (MRSA).

4.                   and fungi like trichophyton interdigitale (athlete’s foot).

information obtained from: http://www.coastwidelabs.com/Technical%20A.../quatbleach.htm

I know this speaks of surface and floor cleaning but, those things come in contact with the same and or simular bacteria and virus pathogens etc... as any item that has come in contact with the human body and air. Does anyone know if I could use it in the ultrasonic machine as the first step of equiptment sterilization? Quats is also non corrosive so you can also soak misc, items in a solution fo extended periods of time without it damaging them. I plan on using this on my surfaces (counters, trays, floors, sinks, and restroom facilities) as I know it is OSHA approved. If anyone has any additional information or opinions I would openly welcome any given. thank-x bunches


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